BioAutomation MerMade 12 Oligonucleotide Synthesizer | 12-Column DNA/RNA/LNA | Used

The BioAutomation MerMade 12 is a column-format oligonucleotide synthesizer built for laboratories that need flexible, medium-throughput DNA, RNA, and LNA synthesis without stepping up to a production-scale platform. Running standard phosphoramidite chemistry, it synthesizes standard, degenerate, and modified oligos — including phosphorothioate backbones via its dedicated sulfurizing line — across a broad scale range in a single run. With 12 independent columns and 16 amidite positions plumbed for both 2′-OMe/DNA and RNA monomers, it is well suited to primer sets, probes, siRNA/antisense work, and gene-assembly building blocks. The MerMade platform has a deep installed base across universities, core facilities, and pharma, making it a proven, serviceable choice for in-house oligo production. This unit ships with an extensive spare-parts and consumables package — solenoid valves, diaphragm valve blocks, bottle-cap/O-ring assemblies, a spare dispense-needle block, gaskets, and cabling — giving a new owner significant built-in maintenance runway.

Condition

Used — inspected. Cosmetic wear consistent with prior laboratory service. Instrument has not been functionally/synthesis tested; No control computer or Poseidon software is included; buyer must supply a compatible PC and licensed control software to operate. Sold as pictured.

Technical Specifications

• Manufacturer: BioAutomation Corporation (MerMade brand, now LGC/Biosearch Technologies)
• Model: MerMade 12
• Type: Automated oligonucleotide synthesizer (DNA / RNA / LNA)
• Chemistry: Standard phosphoramidite; supports standard, degenerate & modified oligos
• Columns: 12 independent synthesis columns (add/remove columns mid-run)
• Amidite positions: 16 (configured for DNA/2′-OMe + RNA monomers)
• Reagent lines: Activator, Cap A, Cap B, Oxidizer, Sulfurizing (thiolation), Deblock, ACN wash, Aux 1/2
• Scale range (per manufacturer spec): 50 nmol – 200 µmol (higher with certain supports)
• Inert gas: Argon supply, 25 PSI max
• Fluidics: The Lee Company chemical-inert solenoid valves; Parker vacuum valves
• Motion system: Parker pneumatic actuator on THK HSR-15R linear guide
• Monitoring: SMC digital source/amidite/reagent pressure + column vacuum displays; Brooks Instrument air flow meter (0–5 LPM)
• Power: 110–120 VAC, 10 A, 50–60 Hz

Included:

• MerMade 12 synthesizer with reagent bottle rail and amber solvent/reagent bottles
• Reagent/amidite tubing and fittings
• Extensive spare-parts & consumables package, including:
• Spare Lee Company solenoid valves (LFVX0515000BB, 24 VDC, 1–30 PSIG)
• Solenoid chemical valves, 2/2-way (P/N AX2001144, MMSVC-3A) — qty 2
• Parker vacuum valves (P/N LQX12-2W24FFFS-901 / V-LQX12-2W2-FFFS-901)
• Molded bottle cap / O-ring assembly kit (P/N AXSK0111)
• Diaphragm valve manifold blocks with ceramic/PTFE seats
• Spare dispense-needle / probe block with silicone gasket
• Spare column-holder blocks, manifold bar with fittings
• Assorted gaskets, O-rings, ferrules, tubing, and data/sensor cables

LT927