Agilent BioTek Synergy Neo2 Hybrid Multi-Mode Microplate Reader (NEO2MB) with Filter Cube Set — Used

Engineered for the screening laboratory, the BioTek Synergy Neo2 delivers exceptional sensitivity and speed across every major detection mode in a single benchtop platform. Its defining feature is Hybrid Technology — a dedicated variable-bandwidth monochromator light path running independently from a high-transmission filter-cube light path — so absorbance, fluorescence, and luminescence assays are each handled by optics optimized for that mode rather than by a compromise design. With kinetic reading speeds as fast as 6 seconds, Synergy Neo2 is a workhorse in the HTS lab. 

This particular instrument is supplied in the NEO2MB configuration (monochromator optics + integrated barcode-labeled filter-cube reader) and comes with an extensive complement of filter cubes — fluorescence intensity, fluorescence polarization (FP), time-resolved fluorescence for HTRF/TR-FRET (including the classic Eu 620/665 donor–acceptor pair), and luminescence including a 1536-well cube. It is an ideal acquisition for drug-discovery, biochemistry, molecular-biology, and academic core facilities that need genuine multi-mode flexibility without a six-figure capital outlay.

Typical applications: ELISA and protein/nucleic-acid quantification, reporter-gene and cell-viability assays, kinase and binding assays (FP), HTRF / TR-FRET, BRET and dual-luminescence, and micro-volume quantification with Take3 plates.

Technical Specifications

This unit:

• Manufacturer: BioTek Instruments (now Agilent), Winooski, VT — Made in USA
• Model / REF: NEO2MB (Monochromator + Barcode-reader configuration)
• Serial No.: 1605181D · Mfg. Date: 18-May-2016
• Power supply: External XP Power AHM250PS24 — Input 100–240 V~, 3.0 A, 50/60 Hz; Output 24 VDC, 10.41 A (250 W)
• Rear I/O: RS-232, USB (Type B), Dispenser Port, Barcode, Gas-Control tubing ports, Mixing-Fan Power
• Marked Class 1 Laser Product (consistent with the integrated barcode-cube reader) · bears US Patent No. 8,218,141

Platform capabilities (Synergy Neo2 series):

• Detection modes: UV-Vis absorbance, fluorescence intensity, luminescence, fluorescence polarization, and time-resolved fluorescence; microplate types from 6- to 1536-well, plus Petri/cell-culture dishes and Take3 micro-volume plates. 
• Quadruple scientific monochromators with continuously variable bandwidth from 3 nm to 50 nm, selectable in 1 nm increments. 
• Temperature control: 4-zone incubation to 65 °C with Condensation Control, ±0.2 °C at 37 °C; shaking: linear, orbital, double-orbital. 
• Operated by Gen5 Microplate Reader software; Gen5 Secure adds 21 CFR Part 11 support; BioStack and 3rd-party automation compatible.

What's Included

• (1) BioTek Synergy Neo2 reader — REF NEO2MB, S/N 1605181D
• (1) XP Power 24 VDC / 250 W external power supply (Model AHM250PS24) with DC cable
• (2) Filter-cube storage racks
• Barcode-labeled filter cube set as pictured (quantities approximate):
• Fluorescence intensity: #102 (400/460 · 400/528), #111 (485/528 · 530/590), #112 (360/620)
• Fluorescence polarization: #4 (Dual FP), #61 (FP 485/530), #62 (FP 530/590), #65 (FP 485/530 · 530/590)
• Time-resolved fluorescence / HTRF: #11 (Ex 330, ×2), #12 (Ex 340), #17 (Ex 360), #41 (Em 620/665, ×2), #42 (Em 495/520)
• Luminescence: #3 (Single PMT), #114 (Lum 1536)

Not included: Gen5 software/license, PC/laptop, reagent injector/dispenser pump module, CO₂/O₂ gas controller module, BioStack/automation, microplates, USB/AC cables.

Condition

Used / Inspected. Instrument is in good cosmetic condition with only minor handling marks consistent with normal prior laboratory use. Filter cubes and optical surfaces appear clean.

LT902-G15