Gilson GX-281 Preparative HPLC Purification System | 333/334 Prep Pumps, 159 UV/VIS, TRILUTION LC, GX Direct Injection — Used / Inspected

This is a complete, bench-ready Gilson preparative HPLC purification workstation built around the VERITY GX-281 liquid handler/fraction collector — the same platform Gilson sells as the VERITY 281 HPLC System. The system is built around the GX-281 liquid handler/fraction collector with a bench-saving footprint and separate fluid pathways for samples and fraction collection, controlled by TRILUTION LC software. It is configured for the full purification cycle — automated injection, gradient separation, UV-triggered fraction collection, and re-injection — in a single integrated package. 

The dual Gilson 333/334 prep pumps form a high-pressure binary gradient system. The 333/334 pumps are designed for preparative HPLC in normal and reverse-phase modes, accommodating columns from 10 to 50 mm ID at flow rates from 1 to 200 mL/min and pressures up to 3,040 psi (210 bar). For isocratic methods the 333 acts as master pump; the 333/334 combination delivers binary gradients, with milligram-to-gram loading up to 15 g depending on column size. Detection is handled by the higher-end Gilson 159 UV/VIS detector: the 159 monitors up to four wavelengths simultaneously while acquiring spectral data from 190–750 nm, with adjustable path-length flow cells accommodating 4.6 to 50 mm ID columns from analytical to pilot scale. Gilson + 2

This is a genuine compound-isolation engine for synthetic, medicinal, and natural-product chemistry — and unlike most secondhand units, this one received a Gilson factory preventive-maintenance service on 05/27/2025 (sticker present, performed by a named Gilson field service specialist).

Condition Notes

Condition: Used / Inspected — Good, powers on. Every module was visually and electrically inspected and powered up:

• 333 & 334 pumps: Power on; master-pump keypad/manometric display is live and reading flow rate, %A composition, and pressure. Pump heads and piston assemblies clean, no visible leakage at the heads.
• 159 UV/VIS detector: Powers on; front display active and reporting normal status (Start/Error/AZ all 0); POWER LED green, ON indicator lit, ERROR clear. Flow cell module installed.
• GX-281 liquid handler: Powers on; X/Y/Z arm, probe, GX Direct Injection Module (VICI valve, LOAD/INJECT), and GX Prep solvent-vent valve all present and intact. Sample bed clean with rack holders and glass collection-tube racks installed.
• 506C System Interface, Dell OptiPlex 7080 PC + TRILUTION LC software: Powers on; TRILUTION LC launches to the Method Builder configuration screen.
• Service status: Gilson PM completed 05/27/2025; next PM nominally due 05/2026.
• Cosmetic: Standard lab wear (minor scuffs/handling marks); no structural damage observed.

Serial / ID numbers captured:

• GX-281 Liquid Handler — Serial/Tag 262E1G091
• 159 UV/VIS Detector — Serial DNA212716003, Product No. E6520XC16
  

Technical Specifications

• System type: Semi-preparative / preparative HPLC purification system (injection, separation, fraction collection, re-injection)
• Pumps: Gilson 333 (master/primary) + 334 (secondary) prep pumps — binary high-pressure gradient. Columns 10–50 mm ID, 1–200 mL/min, up to 3,040 psi (210 bar); variable-volume dynamic mixer; optional four-solvent valve allows up to eight solvents; parallel pumps double flow to 400 mL/min 
• Detector: Gilson 159 UV/VIS — 190–750 nm, up to 4 wavelengths simultaneously, interchangeable/adjustable path-length flow cells, 4.6–50 mm ID columns; 2× 0–5 V scalable analog outputs, Ethernet communication 
• Liquid handler: Gilson VERITY GX-281 — bed holds up to six sample racks (up to 576 × 13-mm tubes), ~33 in (84 cm) of linear bench space, GX Solvent System (syringeless) with 2 µL–100 mL dynamic range and 1 µL/min–25 mL/min flow; arm speed X/Y 0.01–600 mm/s, Z 0.01–125 mm/s; RS-232 or GSIOC, four inputs and four relay outputs 
• Injection: GX Direct Injection Module — injection port integrated into the valve to remove a carryover source, with continuous flow-path design supporting flow rates up to 200 mL/min 
• System interface: Gilson 506C (6 outputs, 4 inputs, 4 analog inputs; RS-232/GSIOC; 12 V)
• Control software: Gilson TRILUTION LC on Dell OptiPlex 7080 — controls the entire system including method screening, scout runs, purification runs, and fraction re-injection 
• Power: Pumps 100–240 V / 600 VA; detector 100–240 V, 75 W (universal, auto-sensing)
• Origin: GX-281 & pumps made in USA; 159 detector made in Germany

Included in this Package

• (1) Gilson VERITY GX-281 Liquid Handler / Fraction Collector with controller/power module
• (1) Gilson 333 prep pump (master, with keypad/manometric display) + (1) Gilson 334 prep pump (secondary)
• (1) Gilson 159 UV/VIS detector with flow-cell module
• (1) Gilson GX Direct Injection Module (VICI 2-position valve) + (1) Gilson GX Prep solvent-vent valve (8-position)
• (1) Gilson 506C System Interface
• (1) Dell OptiPlex 7080 PC with TRILUTION LC software installed, keyboard, mouse, monitor
• Sample-rack holders + glass fraction-collection tube racks; GX tall-drain tubes
• (2+) Phenomenex Gemini-NX C18 AXIA prep columns currently mounted — 100 × 30 mm (P/N 00D-4455-U0-AX) and 150 × 30 mm (P/N 00F-4455-U0-AX), 10 µm/110 Å (used consumables — included as-is, lifetime not warranted)
• Original Gilson accessory kit: GSIOC & RS-232 cables, tubing kits, spare-parts bags, Gilson software USB, and documentation discs (333/334 Pumps, 159 Detector, 506C, GX-281)

LT851